Thiosulfate Citrate Bile Sucrose Agar (TCBS) proposed by NAKANISHI (1962), modified by KOBAYASHI et al. (1963).
Use : isolation and selective cultivation of Vibrio cholera and other enteropahtogenic vibrios
Preparation Suspend 88 g/liter and pour plates. Do not autoclave. pH: 8.6 ± 0.2 at 25°C. The plates are clear and green-blue.
Appearance of colonies microorganisms
Use : isolation and selective cultivation of Vibrio cholera and other enteropahtogenic vibrios
Preparation Suspend 88 g/liter and pour plates. Do not autoclave. pH: 8.6 ± 0.2 at 25°C. The plates are clear and green-blue.
Appearance of colonies microorganisms
Flat, 2-3 mm in diameter, yellow | Vibrio cholerae, Vibrio cholerae type El Tor |
Small, blue-green centre | Vibrio parahaemolyticus |
Large, yellow | Vibrio alginolyticus |
Blue | Pseudomonas, Aeromonas and others |
Very small, translucent | Enterobacteriaceae and others |
Cholera
ReplyDeleteis an acute diarrhoeal disease that can kill within hours if left untreated.
There are an estimated 3–5 million cholera cases and 100 000–120 000 deaths due to cholera every year.
Up to 80% of cases can be successfully treated with oral rehydration salts.