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A blog by MD ASHFAQ AHMED

Wednesday, 4 April 2012

TCBS CULTURE (microbiology)

Thiosulfate Citrate Bile Sucrose Agar (TCBS) proposed by NAKANISHI (1962), modified by KOBAYASHI et al. (1963).

Use :  isolation and selective cultivation of Vibrio cholera and other enteropahtogenic vibrios

Preparation Suspend 88 g/liter and pour plates. Do not autoclave. pH: 8.6 ± 0.2 at 25°C. The plates are clear and green-blue.

Appearance of colonies                                                                      microorganisms

Flat, 2-3 mm in diameter, yellow Vibrio cholerae, Vibrio cholerae type El Tor
Small, blue-green centre Vibrio parahaemolyticus
Large, yellow Vibrio alginolyticus
Blue Pseudomonas, Aeromonas and others
Very small, translucent Enterobacteriaceae and others

1 comments:

  1. Cholera
    is an acute diarrhoeal disease that can kill within hours if left untreated.
    There are an estimated 3–5 million cholera cases and 100 000–120 000 deaths due to cholera every year.
    Up to 80% of cases can be successfully treated with oral rehydration salts.

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